Figure 2
Stable expression of eGFP in the presence of transposase. A: Relative proportion evolution, with respect to time after transfection, of eGFP-positive cells within a population of T2EC, either transfected with the pT2MIK-eGFP expression plasmid only (eGFP), or cotransfected with the pT2MIK-eGFP plasmid and the transposase expression pCAGGS-T2TP plasmid (eGFP + T) with an equimolar ratio (1/1). Significant differences were determined with a t-Test (n ≥ 3): p = 2.44 × 10-2 (*) and p = 9 × 10-4 (* *). B: Relative proportion evolution, with respect to time after transfection, of eGFP-positive cells within a population of T2EC cotransfected with the pT2MIK-eGFP plasmid (eGFP) and the pCAGGS-T2TP plasmid (T). As indicated in the legend, different molecular ratios were tested, pT2MIK-eGFP (7.6 kb)/pCAGGS-T2TP (7 kb): 1/5; 1/1; 5/1 and 10/1, but the amount of total DNA (pT2MIK-eGFP + pCAGGS-T2TP) was constant. For one given time point, conditions not connected by the same letter are significantly different, p < 0.05 (Tukey-Kramer HSD test, n ≥ 3). Cell fluorescence was analyzed by flow cytometry (FACS). The positive fluorescence threshold is fixed in order to have 99% of the negative cells (i.e. cells transfected with the empty plasmid) below this threshold. A 1% value is, hence, considered as null.