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Table 2 Effect of physical treatments on the stability of purified TAT-Cre recombinase

From: Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo

 

DLS

(RH in nm)

AI

(280em/340em nm)

Control

3.98 - monodispersed

0.02

30' at 20°C

3.77 - monodispersed

0.04

60' at 20°C

3.87 - monodispersed

0.04

4 h at 20°C

3.94 - monodispersed

0.06

30' at 30°C

4.01 - monodispersed

0.06

10' at 40°C

3.96 - monodispersed

0.06

Sonication at 4°C

polydispersed

10

Sonication at 20°C

polydispersed

14

Sonication at 4°C + Trehalose

polydispersed

3.7

Sonication at 4°C + NiCl2

polydispersed

46.7

Sonication at 4°C + TA

polydispersed

2.5

Sonication at 4°C + NiCl2 + TA

polydispersed

4.7

  1. The effect of increasing temperature, sonication, trehalose (50 mM), NiCl2 (1 mM), and tartaric acid (200 mM) on the enzyme monodispersity was analyzed by dynamic light scattering (DLS) and fluorimetric aggregation index (AI) using purified recombinant protein samples (50 μg/mL). DLS: the hydrodynamic radius (RH) of the molecules in solution is expressed in nm; the AI is the ratio between the light scattering at 280 nm and the emission at 340 nm. Experiments have been performed in triplicate.
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BMC Biotechnology

ISSN: 1472-6750

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