From: Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo
DLS (RH in nm) | AI (280em/340em nm) | |
---|---|---|
Control | 3.98 - monodispersed | 0.02 |
1 mM NiCl2 at 4°C | polydispersed | 8.5 |
10 mM NiCl2 at 4°C | polydispersed | 9.2 |
1 mM NiCl2 at 20°C | - | precipitated |
1 mM NiCl2 at 30°C | - | precipitated |
0.1 mM NiCl2 at 4°C | polydispersed | 3.1 |
0.01 mM NiCl2 at 4°C | 4.15 - monodispersed | 0.4 |
1 mM NiCl2 + 5 mM EDTA | polydispersed | 3.3 |
1 mM NiCl2 + 10 mM EDTA | polydispersed | 2.2 |
1 mM NiCl2 + 200 mM TA | 3.92 - monodispersed | 0.02 |