Figure 5
From: Monodispersity of recombinant Cre recombinase correlates with its effectiveness in vivo
Effect of chloroquine and sucrose addition on the TAT-Cre release from endosomes. The β-catenin expression silencing induced by 100 μg/mL TAT-Cre in the presence of increasing concentrations of chloroquine (0-100 μM) and sucrose (0-500 μM) was evaluated by immunofluorescence. Similarly, the Cre recombinase activity of increasing TAT-Cre concentrations (0-100 μg/mL) was examined in the presence of 100 μM chloroquine.