Figure 4

Analysis of scFv AB28 expression in tobacco leaves. (a, b) Reducing 12% SDS-PAGE analysis of leaf discs: Coomassie staining (a) and Western blot with detection using anti-His antibody (b). Lanes: M, molecular mass markers (values in kDa are shown on the left); 1-5, leaf materials after expression of scFv AB09, AB28, AD10, AA28 and AB21, respectively; 6,7, negative controls (tobacco expressing GFP and wt plants, respectively). (c,d) Characterization of tobacco expressed scFv AB28 after purification. (c) Coomassie stained 12% SDS-PAA gel. Lanes: M, molecular mass markers; 1-4, scFv samples (1.3, 2.6, 5.2 and 13 μg, respectively). (d) Analysis of scFv AB28 molecular forms by size-exclusion FPLC on a calibrated Superdex 200 column. The positions of the molecular weight markers are indicated.