Figure 3

Analyses of specificity of sporozoite-neutralizing scFvs. (a) Western blot analysis of antigen specificity of scFv AB28. Lanes: M, molecular mass markers (values in kDa are shown on the left); 1,2, produced in E. coli 25 kDa precursor of a GPI-anchored surface antigen EtSAG1 under reducing and non-reducing conditions, respectively; 3,4, oocyst extract of E. tenella under non-reducing and reducing conditions, respectively. (b) Species specificity of scFv AA28, as determined by ELISA using oocyst extracts from three avian (E. tenella, E. acervulina, E. brunetti) and two rodent (E. papillata and E. nieschulzi) Eimeria species. As a negative control, secondary anti-c-myc tag antibody followed by HRP-conjugated goat anti-mouse IgG antibody was used.