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Table 4 Validation of predicted assay performance on cDNA (Molecules measured from the cDNA equivalent of 10 nanograms of total RNA from secondary phloem).

From: Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR

  Assay 1 (Quantitect, 2 min A&E) Primer Pairs Assay 2 (Quantifast, 1 min A&E) Primer Pairs
Tree number (genotype) T1-C2 G1-A2 G1-C2 T1-C2 G1-A2 G1-C2
Tree-14 (G-T/G-G) 1100 16000 18000 17000 53000 47000
Tree-17 (G-T/G-G) 1400 23000 29000 22000 54000 31000
Tree -20 (G-T/G-G) 1500 32000 21000 30000 81000 74000
Mean ± SD 1400 ± 200 24000 ± 8000 23000 ± 6000 23000 ± 7000 63000 ± 16000 51000 ± 22000
Tree-2 (G-T/G-T) 18 24000 380 11000 27000 20000
Tree-4 (G-T/G-T) 67 51000 1100 15000 35000 25000
Tree-6 (G-T/G-T) 69 54000 630 23000 60000 47000
Mean ± SD 51 ± 29 43000 ± 16000 690 ± 30 17000 ± 6000 41000 ± 17000 31000 ± 14000