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Table 3 PCR assay properties.

From: Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR

Properties Assay 1 Assay 2
Master mix Quantitect Quantifast
A&E temperature 62°C 62°C
A&E time 2 min 1 min
Priming probabilities       
  99% Confidence interval 1 99% Confidence interval 1
  Lower Mean Upper Lower Mean Upper
T1-C2 on T-G (PM) 0.39 0.79 1.6 0.48 1.1 2.3
G1-C2 on G-G (PM) 0.49 1.0 2.1 0.50 0.95 1.8
G1-A2 on G-T (PM) 0.31 0.70 1.5 0.52 0.91 1.5
T1-C2 on G-T (2) 0.0000032 0.00021 0.0011 0.21 0.51 1.2
T1-C2 on G-G (1) 0.0090 0.015 0.025 0.41 1.2 3.5
G1-C2 on T-G (1) 0.048 0.085 0.15 0.34 0.79 1.8
G1-C2 on G-T (1) 0.0019 0.0061 0.019 0.28 0.65 1.5
G1-A2 on T-G (2) 0.00034 0.0030 0.025 0.25 0.83 2.7
G1-A2 on G-G (1) 0.0026 0.020 0.16 0.51 1.1 2.4
  1. 1Three standard deviations above (upper) or below (lower) the mean. Means and intervals were calculated using log2 transformed data.