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Table 3 PCR assay properties.

From: Evaluation of the impact of single nucleotide polymorphisms and primer mismatches on quantitative PCR

Properties

Assay 1

Assay 2

Master mix

Quantitect

Quantifast

A&E temperature

62°C

62°C

A&E time

2 min

1 min

Priming probabilities

      
 

99% Confidence interval 1

99% Confidence interval 1

 

Lower

Mean

Upper

Lower

Mean

Upper

T1-C2 on T-G (PM)

0.39

0.79

1.6

0.48

1.1

2.3

G1-C2 on G-G (PM)

0.49

1.0

2.1

0.50

0.95

1.8

G1-A2 on G-T (PM)

0.31

0.70

1.5

0.52

0.91

1.5

T1-C2 on G-T (2)

0.0000032

0.00021

0.0011

0.21

0.51

1.2

T1-C2 on G-G (1)

0.0090

0.015

0.025

0.41

1.2

3.5

G1-C2 on T-G (1)

0.048

0.085

0.15

0.34

0.79

1.8

G1-C2 on G-T (1)

0.0019

0.0061

0.019

0.28

0.65

1.5

G1-A2 on T-G (2)

0.00034

0.0030

0.025

0.25

0.83

2.7

G1-A2 on G-G (1)

0.0026

0.020

0.16

0.51

1.1

2.4

  1. 1Three standard deviations above (upper) or below (lower) the mean. Means and intervals were calculated using log2 transformed data.
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BMC Biotechnology

ISSN: 1472-6750

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