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Table 1 Lethal dose response data derived from topical bioassays of the parent M. anisopliae ARSEF2575, EVG016 and EVG017g strains with adult M. sanguinipes grasshoppers at 28°C.

From: Directed evolution of a filamentous fungus for thermotolerance

M.a. strain Assay LD50 95% CL Slope (SE) Chi Sqr LD95 95% CL
2575 1 799 63–1,722 1.46 (0.51) 0.04 10,599 4,415–36,196
  2 1,815 1,174–3,042 1.60 (0.24) 1.55 19,503 9.279–68,978
  mean (S.D.) 1,307 (718)     15,051 (6,296)  
EVG016 1 25,453 19,600–41,000 3.73 (0.82) 0.75 70,257 50,534–138,856
  2 19,758 14,000–2.7400 2.55 (0.37) 2.89 87,347 57,234–169,968
  mean (S.D.) 22,605 (4,027)     78,802 (12,084)  
EVG017g 1 8,939 4,425–13,194 2.50 (0.64) 0.71 40,787 25,601–127,114
  2 14,007 4,365–25,838 1.62 (0.41) 2.35 145,180 71,373–765,860
  mean (S.D.) 11,473 (3,584)     92,983 (73,817)  
  1. Units for LD and confidence levels: conidia/insect. Data are derived from two replicate bioassays using a total of 120–150 insects/bioassay.