Directed evolution of a filamentous fungus for thermotolerance

BMC Biotechnology

Table 1 Lethal dose response data derived from topical bioassays of the parent M. anisopliae ARSEF2575, EVG016 and EVG017g strains with adult M. sanguinipes grasshoppers at 28°C.

M.a. strain	Assay	LD₅₀	95% CL	Slope (SE)	Chi Sqr	LD₉₅	95% CL
2575	1	799	63–1,722	1.46 (0.51)	0.04	10,599	4,415–36,196
	2	1,815	1,174–3,042	1.60 (0.24)	1.55	19,503	9.279–68,978
	mean (S.D.)	1,307 (718)				15,051 (6,296)
EVG016	1	25,453	19,600–41,000	3.73 (0.82)	0.75	70,257	50,534–138,856
	2	19,758	14,000–2.7400	2.55 (0.37)	2.89	87,347	57,234–169,968
	mean (S.D.)	22,605 (4,027)				78,802 (12,084)
EVG017g	1	8,939	4,425–13,194	2.50 (0.64)	0.71	40,787	25,601–127,114
	2	14,007	4,365–25,838	1.62 (0.41)	2.35	145,180	71,373–765,860
	mean (S.D.)	11,473 (3,584)				92,983 (73,817)

Units for LD and confidence levels: conidia/insect. Data are derived from two replicate bioassays using a total of 120–150 insects/bioassay.

ISSN: 1472-6750