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Figure 7 | BMC Biotechnology

Figure 7

From: Transduction of the MPG-tagged fusion protein into mammalian cells and oocytes depends on amiloride-sensitive endocytic pathway

Figure 7

Successful transduction of MPG-EGFP into the mouse oocytes. Mature oocytes were obtained from 4-week mouse ovaries, and zona pellucidae were removed by the acid Tyrode solution. EGFP or MPG-EGFP was added to M16 media for 1 hr. Note the strong peripheral EGFP signal in MPG-EGFP treated oocytes. Amiloride, as in HeLa cells, effectively blocked the transduction of MPG-EGFP.

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BMC Biotechnology

ISSN: 1472-6750

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