Figure 3

Sensitivity assessment of LAMP Detection. A. Sensitivity of LAMP using genomic target. DNA from MS8/RF3 sample (16 ng.μL^-1) was serially diluted and amplified by LAMP, in triplicate, using primers to assay for the RF3 junction. The numbers in parenthesis represent the approximate copy numbers of the target assuming that the sample represents RF3 in a hemizygous state (determined using RT-PCR data not shown) for the transgene and using 1 pg as the genome size for oilseed rape. C is the no DNA control and M represents molecular size markers. The smear (*) shows an example of non-specific amplification. B. Sensitivity of LAMP using plasmid target. Serial dilutions of the plasmid pGreenII were amplified using primers for the Pnos target. Numbers represent the calculated copy numbers of the plasmid derived from the DNA value. C is the no DNA control and M represents molecular sized markers.