Evaluation of cross-linked aggregates from purified Bacillus subtilislevansucrase mutants for transfructosylation reactions

Table 3 Characterization of WT, R360K and Y429N LS immobilized on Eupergit C^®.

Enzyme	mg LS/g Eupergit C^®	Immobilization yield (%)^d	U LSs/mg EupergitC^®
WT LS^a	6.8	59	0.64
Y429N^a	7.8	55	0.006
R360K^a	7.1	60	0.74
R360K^b	9.3	58	0.93
R360K^c	18.8	51	1.64

^a Immobilization at pH 6.0, during 38 h.^b Immobilization at pH 7.0, during 38 h. Immobilization at pH 7.0, during 72 h. In all cases 10 mg of enzyme were contacted per g of Eupergit C^®, except for (c), where 20 mg were used.^dYield is defined as the activity recovered in Eupergit C^® when compared with the total activity load of free enzyme in the assay (100%). Eupergit is reported in dry basis.

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