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Table 2 Characterization of Levansucrases-CLEAs

From: Evaluation of cross-linked aggregates from purified Bacillus subtilislevansucrase mutants for transfructosylation reactions

Enzyme

Aggregation yielda (%)

LS-CLEAs

U/mg of CLEA

WT LS

28 (± 4.2)

44.9 (± 6.9)

R360K

33 (± 4.1)

56.5 (± 7.2)

Y429N

80 (± 7.5)

1.2 (± 0.1)

  1. a Aggregation yield is defined as the activity recovered in CLEAs when compared with the total activity load of soluble enzyme in the experiment (100%).
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BMC Biotechnology

ISSN: 1472-6750

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