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Figure 3 | BMC Biotechnology

Figure 3

From: Evaluation of cross-linked aggregates from purified Bacillus subtilislevansucrase mutants for transfructosylation reactions

Figure 3

Reaction evolution during oligosaccharides/levan (A) and fructosyl-xyloside (B) synthesis. Free R360K (rhombus), WT-CLEAs (circles), R360K-CLEAs (inverted triangles) and Y429N-CLEAs (squares). Black symbols indicate sucrose consumption, while open symbols represent the fructosyl-xyloside synthesis. WT and R360K-CLEAs undergo diffusional restrictions when sucrose is used both as donor and acceptor due to the levan synthesis (A), while, in the presence of xylose, all LS-CLEAs synthesize efficiently the fructosyl-xyloside (B). Reactions for oligosaccharides/levan and fructosyl-xyloside synthesis were carried out with 0.1 and 0.5 U/mL of enzymatic activity respectively. Results represent the mean (± SD) of three experiments.

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