Figure 1From: Evaluation of cross-linked aggregates from purified Bacillus subtilislevansucrase mutants for transfructosylation reactionsEffect of glutaraldehyde concentration on relative CLEAs activity. WT-CLEAs (black circles), R360K-CLEAs (black inverted triangles) and Y429N-CLEAs (black squares). The effect of cross-linking was examined in the range of 0.2 to 1% glutaraldehyde (v/v), using 1 mg/mL of purified enzymes. Activity after cross-linking was compared to the activity of soluble enzymes, which was taken as 100%. Results represent the mean (± SD) of five experiments.Back to article page