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Figure 5 | BMC Biotechnology

Figure 5

From: Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342

Figure 5

Microscopy-based sensitivity analysis of PlyG-P3 peptide binding. Bacterial cultures of Bacillus cereus 4342, B. anthracis-Sterne, B. cereus-11778, B. thuringiensis, and B. cereus-10876 were incubated with PlyG-P3 and the complex was detected by incubating with strepatividin-conjugated quantum dots. Peptide binding was detected by the fluorescing Q dots using appropriate UV filters (605 nm) under a Nikon microscope, either in phase-contrast (panels on left) or fluorescence (panels on right) fields. Note that even a single bacterium is visible in the B. cereus-Sterne field.

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