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Figure 4 | BMC Biotechnology

Figure 4

From: Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342

Figure 4

Streptavidin-conjugated Quantum Dot (QD) based analysis of PlyG peptides binding to bacteria. Human plasma was spiked with B. cereus-4342, B. anthracis-Sterne, B. cereus-11778, B. thuringiensis and B. cereus-10876. Each PlyG peptide (P1-P6) were incubated with the bacteria. Following incubation, the samples were centrifuged and resuspended in PBS. Streptavidin-conjugated quantum-dots (QDs) were then added to the sample (bacteria-peptide complex) and analyzed by a fluorescence plate reader (Synergy 4TM, Biotek, USA) with excitation spectrum set at 360–485 nm and emission spectrum at 605 nm. Error bars indicate the SD from 3 independent experiments. Statistically significant difference (P < 0.05) is represented by (*).

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BMC Biotechnology

ISSN: 1472-6750

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