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Figure 3 | BMC Biotechnology

Figure 3

From: Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342

Figure 3

ELISA-based analysis of PlyG peptide binding to B. cereus- 4342, B. anthracis -Sterne, B. cereus -11778, B. thuringiensis , and B. cereus -10876. Individual wells of a 96-well microplate were coated with desired bacterial suspensions (103 CFU) and incubated with biotinylated PlyG peptides prior to incubation with streptavidin-HRP. Color was developed by adding TMB. Optical density of the reactions were measured at 490 nm using a microplate reader. Error bars indicate the SD from three independent experiments. Statistically significant difference (P <0.05) is represented by (*).

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BMC Biotechnology

ISSN: 1472-6750

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