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Figure 4 | BMC Biotechnology

Figure 4

From: Efficient, chemoselective synthesis of immunomicelles using single-domain antibodies with a C-terminal thioester

Figure 4

Chromatogram and deconvoluted mass spectrum of the biotinylated single-domain antibody against GST after overnight ligation. pelB-biotin: biotinylated sdAb-aGST with pelB leader attached (calcd. mass 19879 Da); pelB: MESNA thioester of the sdAb-aGST with pelB leader attached (calcd. mass 19632 Da); pelBΔ3-biotin: biotinylated sdAb-aGST with the first three amino acids of the pelB leader removed (calcd. mass 19457 Da);); pelBΔ14-biotin: biotinylated sdAb-aGST with the first 14 amino acids of the pelB leader removed (calcd. mass 18423 Da);pelBΔ15-biotin: biotinylated sdAb-aGST with the first 15 amino acids of the pelB leader removed (calcd. mass 18309 Da); pelBΔ16-biotin: biotinylated sdAb-aGST with the first 16 amino acids of the pelB leader removed (calcd. mass 18238 Da);

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