Table 2 Most successful combination of parameters for G. pectorale transformation using a PDS-1000/He biolistic device.

material of microprojectiles

gold

size of microprojectiles

0.6 μm in diameter

selectable marker plasmid

pPmr3

coating of microprojectiles

plasmid-DNA/microcarrier/CaCl₂/spermidine/EtOH-precipitation

target cells

immobilized on cellulose acetate membrane filter; almost free of liquid

burst pressure of rupture disks

1100 psi

rupture disk-macrocarrier distance

7 mm

macrocarrier-stopping screen distance

8 mm

stopping screen-target cell distance

6 cm

chamber evacuation

27 inch Hg

cultivation after particle bombardment

in liquid medium