Figure 4From: Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteinsInfluence of initial protein concentration on the amount of immobilized protein. Results are from a 10-h, direct-capture adsorption of GST-Neu5Ac aldolase-5R (a) or GST-Neu5Ac aldolase-5R (b), from crude protein extracts. In each experiment, 10 ml of protein solution was incubated with 1.2–1.3 g (wet weight) of ion-exchange resin for 10 h.Back to article page