Figure 3From: Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteinsImmobilization of purified double-tagged GST-Neu5Ac aldolase-5R using cationic exchanger SP Sepharose. The amount of fusion protein immobilized varies with the amount of fusion protein used. Prior to immobilization, GST-Neu5Ac aldolase-5R was purified using a GSH-affinity column. Data are an average of four independent experiments.Back to article page