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Figure 2 | BMC Biotechnology

Figure 2

From: Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins

Figure 2

SDS-PAGE analysis and Western blotting of GST-Neu5Ac aldolase-5R. For panel (a), samples are the supernatant (S) and precipitate (P) of cell extracts from cultures induced with 0.01, 0.1 or 1 mM IPTG at 28°C for 7 h. In panel (b) and (c), samples are fractions collected from a GSH-affinity column. Lane M, molecular size makers; lane 1–7, fractions 1–7. Arrows indicate the purified fusion proteins.

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BMC Biotechnology

ISSN: 1472-6750

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