Skip to main content
Figure 1 | BMC Biotechnology

Figure 1

From: Production of N-acetyl-D-neuraminic acid using two sequential enzymes overexpressed as double-tagged fusion proteins

Figure 1

SDS-PAGE analysis and activity assay of GST-GlcNAc 2-epimerase-5D. (a) Samples are the supernatant (left lanes) and precipitate (right lanes) of cell extracts from cultures induced with different concentrations of IPTG (0.01, 0.1 and 1 mM) at 28°C for 7 h. (b) Specific activity in the supernatant and precipitate of cell extracts from cultures induced with 0.01 mM IPTG at 28°C for 0 to 7 h. The enzymatic activity of GlcNAc 2-epimerase was based on the formation of GlcNAc from ManNAc. SDS-PAGE analysis of proteins in the supernatant (c) and precipitate (d) of cell extracts from cultures at different induction times is also shown. M denotes marker proteins; arrows indicate the positions of fusion proteins.

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us