Table 4 Comparison of the single-primer and double-primer PCR reactions.

Number of reactions

2 (1 for each primer)

1

Plasmid template

~500 ng

5–50 ng

Primers

Forward OR reverse

(each in a separate tube)

Forward AND reverse together

Primer-primer annealing during PCR

-

+

Number of cycles

30

12–18

Fold amplification of DNA

30

4,096–262,144

Probability of mutation during PCR

Low*

High*

DNA polymerase

Pwo

PfuTurbo