Single-primer PCR | Double-primer PCR | |
---|---|---|
Number of reactions | 2 (1 for each primer) | 1 |
Plasmid template | ~500 ng | 5–50 ng |
Primers | Forward OR reverse (each in a separate tube) | Forward AND reverse together |
Primer-primer annealing during PCR | - | + |
Number of cycles | 30 | 12–18 |
Fold amplification of DNA | 30 | 4,096–262,144 |
Probability of mutation during PCR | Low* | High* |
DNA polymerase | Pwo | PfuTurbo |