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Table 3 Denaturation and slow cooling conditions to allow reannealing of PCR products.

From: Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

Step

Temperature (°C)

Time

(min)

1

95

5

2

90

1

3

80

1

4

70

0.5

5

60

0.5

6

50

0.5

7

40

0.5

8

37

Holding

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BMC Biotechnology

ISSN: 1472-6750

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