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Table 3 Denaturation and slow cooling conditions to allow reannealing of PCR products.

From: Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

Step Temperature (°C) Time
(min)
1 95 5
2 90 1
3 80 1
4 70 0.5
5 60 0.5
6 50 0.5
7 40 0.5
8 37 Holding