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Figure 3 | BMC Biotechnology

Figure 3

From: Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies

Figure 3

Separation of DNA products after Single Primer PCR and DpnI digestion reactions. Plasmid: Parental plasmid alone; F: PCR product with a-D338A Forward primer; R: PCR product with a-D338A Reverse primer; +DpnI: Forward and Reverse primer PCR products combined and digested with DpnI. DNA samples were purified using QIAquick purification kit (Qiagen) and electrophoresed in 1% agarose with Tris-acetate (40 mM Tris, 20 mM sodium acetate, 1 mM EDTA, pH 8.0) as the running buffer. Ethidium bromide stained gel is shown in inverted color (black to white), without any additional digital image editing. The 1 kb DNA ladder marker was from Bioneer (Korea).

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BMC Biotechnology

ISSN: 1472-6750

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