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Table 4 Localisation and evaluation of differences between 43RCA framework regions, and those coded by 43RCA most similar human germline genes

From: Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity

  Total Identical Very similar
(+++)
Similar
(+-+), (++-), (+-+)
Dissimilar
(--+), (-+-), (+- -)
Very dissimilar
(- - -)
VH       
FR1-IMGT 25 23 0 2 0 0
FR2-IMGT 17 14 0 1 0 2
FR3-IMGT 38 34 0 1 3 0
FR4-IMGT 11 9 0 1 0 1
FR-IMGT 91 80 0 5 3 3
V-KAPPA       
FR1-IMGT 26 24 1 1 0 0
FR2-IMGT 17 15 0 0 2 0
FR3-IMGT 36 33 0 1 1 1
FR4-IMGT 10 10 0 0 0 0
FR-IMGT 89 82 1 2 3 1
All FR-IMGT VH and V-KAPPA 180 162 1 7 6 4
  (%) 90% 0.56% 3.89% 3.33% 2.22%
  1. Differences between 43RCA VH and VL framework amino acids, and those coded by most similar human genes, are evaluated on a 5-level scale (from identical to very dissimilar, see text) and located in each framework region.