Table 4 Localisation and evaluation of differences between 43RCA framework regions, and those coded by 43RCA most similar human germline genes
From: Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity
| Â | Total | Identical | Very similar (+++) | Similar (+-+), (++-), (+-+) | Dissimilar (--+), (-+-), (+- -) | Very dissimilar (- - -) |
|---|---|---|---|---|---|---|
VH | Â | Â | Â | Â | Â | Â |
FR1-IMGT | 25 | 23 | 0 | 2 | 0 | 0 |
FR2-IMGT | 17 | 14 | 0 | 1 | 0 | 2 |
FR3-IMGT | 38 | 34 | 0 | 1 | 3 | 0 |
FR4-IMGT | 11 | 9 | 0 | 1 | 0 | 1 |
FR-IMGT | 91 | 80 | 0 | 5 | 3 | 3 |
V-KAPPA | Â | Â | Â | Â | Â | Â |
FR1-IMGT | 26 | 24 | 1 | 1 | 0 | 0 |
FR2-IMGT | 17 | 15 | 0 | 0 | 2 | 0 |
FR3-IMGT | 36 | 33 | 0 | 1 | 1 | 1 |
FR4-IMGT | 10 | 10 | 0 | 0 | 0 | 0 |
FR-IMGT | 89 | 82 | 1 | 2 | 3 | 1 |
All FR-IMGT VH and V-KAPPA | 180 | 162 | 1 | 7 | 6 | 4 |
| Â | (%) | 90% | 0.56% | 3.89% | 3.33% | 2.22% |