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Table 3 Sequence diversity of antibody fragments selected against four different antigens

From: A compact phage display human scFv library for selection of antibodies to a wide variety of antigens

Clone

Family

CDR3

Germline

Amino acids differences from germline

VH gene

    

Aflatoxin C3

VH1

ADDYGSGSYGFDY

IGHV1-3*01 (DP25)

1

Aflatoxin C5

VH3

SRVGLWGPRYYYYYGMDVW

IGHV3-23*04 (DP47)

4

Aflatoxin D2

VH1

GGPLDY

IGHV1-45*02 (DP4)

5

Rabies D7

VH1

GGNFDY

IGHV1-18*01 (DP14)

2

Rabies B5

VH3

GYATFDY

IGHV3-23*01 (DP47)

7

Cobra D11

VH4

HGRDTSGYTMDYFDS

IGHV4-59*07 (H4)

14

Carcinoma C4

VH3

DRGKYPGDGMGV

IGHV3-23*01 (DP47)

6

V L gene

    

Aflatoxin C3

VK1

QQSYSTPYA

IGKV1D-39*01 (DPK9)

4

Aflatoxin C5

VL3

QVWDRDSRTIV

IGLV3-9*01 (V2-6)

16

Aflatoxin D2

VL2

SSYAGSNNLV

IGLV2-8*01 (V1-2)

3

Rabies D7

VL1

AAWDDSLSGPV

IGLV1-47*01 (DPL3)

2

Rabies B5

VK1

QQYSYNPYT

IGKV1D-39*01 (DPK9)

1

Cobra D11

VL6

QSYDSSNRV

IGLV6-57*01 (V1-22)

5

Carcinoma C4

VL1

AAWDDSLNGYV

IGLV1-44*01 (DPL2)

2

  1. * CDR3 sequence and V-gene segment usage for both heavy and light chains of the selected antibody are reported. Sequence analysis was done by Ig BLAST [36] and DNA Plot program [34] (shown in parenthesis).