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Table 2 Results of affinity selections with different targets

From: A compact phage display human scFv library for selection of antibodies to a wide variety of antigens

Antigens Round of panning Number of clone after 1st round Number of binding phage after 1st round Number of binding phage after 2nd round Number of soluble scFv fragments Number of different scFv producing clone
Aflatoxin 1 2.5 × 102 4/56 - 3/4 3/3
Attenuated Rabies viruses 2 1.2 × 103 1/96 7/96 8/8 2/8
Amylase 1 8.8 × 10 3/88 - 3/3 NA
BSA 1 2.0 × 103 6/96 - NA NA
Cobra snake venom 2 1.4 × 104 1/96 9/96 3/3 1/10
Cholangiocarcinoma cell KKU-100 1 2.2 × 103 2/96 - NA 2/2
Green pit viper venom 2 5.1 × 104 0/96 0/96 NA NA
GST 1 4.3 × 102 6/28 - NA NA
  1. * The number of positive clones/the number of screened clones.