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Table 1 Primer sets used in PCR/rtPCR reactions.

From: Novel sequential ChIP and simplified basic ChIP protocols for promoter co-occupancy and target gene identification in human embryonic stem cells

Promoter

Primer sequences (Forward/Reverse)

Reference

NANOG

GTCTTTAGATCAGAGGATGCCCC/CTACCCACCCCCTATTCTCCCA

[14]

c-MYC

GAAGCCTGAGCAGGCGGGGCAGG/GCTTTGATCAAGAGTCCCAG

[15]

BCL-X

CTGCACCTGCCTGCCTTTGC/GGAGAGAAAGAGATTCAGGA

[16]

P21

CCAGCCCTTGGATGGTTT/GCCTCCTTTCTGTGCCTGA

[17]

SUZ12

TCACCCTACCCTGGCCTCGCT/TCGCTAAACCGCTCGCTGGGT

[18]

MUC4

AAACTAGGGACTCCTACTTG/GGACAGAATGGGGTGAAT'

[19]

FOS

GGCGAGCTGTTCCCGTCAATCC/GCGGGCGCTCTGTCGTCAACTCTA

[20]

HOXC13

TGCAGCGGAGCGAGCCCC/TCAACAGGGATGAGCGCGTCGTG

[21]

GLI1

CTCGCGGGTGGTCCGGGCTTG/CCGCCTGCCCCCCCTTCTCA

[22]

BCL2

CAGTGGGTGGCGCGGGCGGCA/CCCGGGAGCCCCCACCCCGT

[23]

E-CADHERIN (CDH1)

TAGAGGGTCACCGCGTCTAT/TCACAGGTGCTTTGCAGTTC

[24]

OCT3/4

TGAACTGTGGTGGAGAGTGC/AGGAAGGGCTAGGACGAGAG

[14]

FGF4

GGGAGGCTACAGACAGCAAG/CTGTGAGCCACCAGACAGAA

[14]

LEFTY

AAGCTGCAGACTTCATTCCA/CGGGGGATAGATGAAGAAAC

[14]

VEGF

CCTCAGTTCCCTGGCAACATCTG/GAAGAATTTGGCACCAAGTTTGT

[25]

SNAIL

GGCGCACCTGCTCGGGGAGTG/GCCGATTGCCGCAGCA

[26]

PTEN

CCGTGCATTTCCCTCTACAC/GAGGCGAGGATAACGAGCTA

[27]

SMA

AGCCAAGCACTGTCAGGA/ACAATGGATGGGAAAACAG

[28]

COL2A

TTCCAGATGGGGCTGAAAC/ATTGTGGGAGAGGGGGTCT

[29]

GATA4

ACAGGAGATGGGAAGTGTCGC/GGTGACCTCTTGGGCTCAACTC

[30]

GATA6

CATTTCCAGTCCCTTTTGCCC/TTCCACATCAGTCGTGTCCGAG

[30]

BAX

ACAGTGGCTCACGCCTGTAAT/AGCCTCCCAAGTAGCTGGAATTG

[31]

TGFB

GTGCAGCAAAAGAGGCTGCGTGCG/TCTATTTCTCTCTGCTGAAAT

[32]

B2R

GCAGAGCGGAGAGCGAAGG/GCCTGATGTCCCCACCGTC

[33]

IL2

CGTTAAACAGTACCTCAAGCTCAA/CCTTTTTATCCACACAAAGAGCTA

[34]

ZFP206

CCGGCCAGATTTCACTAAAGAGC/CCTACCCCATGAAATTTTGCCAG

[35]

GAPDH

GTGTTCCTACCCCCAATGTGT/ATTGTCATACCAGGAAATGAGCTT

[14]

HESX1

GTGTTCATTGACATGCTAA/GGACCAGAAGAAAGACTGTG

[36]

mouse Lama1*

CCTCAGCTCCAAGAAAGGAG/AGGATGCTTCCCTGAAATCC

[37]

  1. * All others are human genes.