Figure 8
From: Interfering ribonucleic acids that suppress expression of multiple unrelated genes
Analysis of target site selection. (a) VEGF-A suppression by 21 nt RNA duplexes (with central mismatches) targeting different regions of the VEGF-A mRNA. ARPE-19 cells were transfected with 40 nM indicated RNA duplexes. VEGF-A in cell culture supernatant was measured by ELISA 48 hours post-transfection and 24 hours post-stimulation with 130 μM Deferoxamine. Each point represents the mean of triplicate samples. Error bars indicate standard deviation. (b) Statistical analysis of data for VEGF-A suppression depicted in Panel (a). There was a significant difference (p < 0.05) between the means for molecules targeting the 3'UTR as compared to molecules targeting the ORF as determined by one-way ANOVA with a Bonferroni post-test.