Figure 7
From: Interfering ribonucleic acids that suppress expression of multiple unrelated genes
Suppression of VEGF-A and ICAM-1 expression by variants (shifted 5' of the guide strand) of CODEMIR-1. ARPE-19 cells were transfected with 40 nM of the indicated duplex RNA. 24 hours after transfection cells were stimulated with 130 μM Deferoxamine (VEGF-A) or 1 ng/ml IL-1β (ICAM-1). Gene expression was assayed 48 hours after transfection by ELISA on cell supernatant (VEGF-A) or FACS (ICAM). All data points indicate the mean of triplicate samples. Error bars indicate standard deviation.