Figure 4

Comparison of CODEMIR-1 variants with (#56 and #76) and without (#120 and #121) 7 G motifs. (a) ARPE-19 cells were transfected with 40 nM duplex RNA and VEGF (ELISA) or ICAM (FACS) were assayed 48 hours post-transfection (*p < 0.001, **p < 0.01 as compared to CODEMIR-56; † p < 0.01, †† p < 0.05 as compared to CODEMIR-76). (b) Normalised fluorescence of ARPE-19 cells transfected with AmCyan/CODEMIR-1 reporter and AsRed plasmids and CODEMIR variants with and without 7 G motifs. ARPE-19 cells were co-transfected with 1 μg of the plasmids and 40 nM indicated RNA duplexes. Fluorescence was assessed by FACS 48 hours post-transfection (*p < 0.001 as compared to CODEMIR-56, **p < 0.001 as compared to CODEMIR-76). Each bar represents the mean of triplicate samples. Error bars indicate standard deviation. Statistical significance was determined by two-way ANOVA using a Bonferroni post-test.