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Table 3 Comparison of the molecular characteristics between native Xyn2 and recombinant Xyn2

From: Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastorisand functional characterization of the produced enzyme

 

Value for Xyn2 from

 

Property

T. reesei

S. cerevisiae

P. pastoris

E. coli

Reference

Expression level (nkat/ml)

54001

1470

4350

445

[7, 8, 12]

Mol. mass (kDa)

20–21

27

21

24

[7, 8, 12]

Optimum pH

5

6

6

5

[7, 8, 12]

pH stability2

4.5–5.0

4.0–7.0

3.0–8.0

3.5–7.5

[7, 8, 12]

Optimum temp (°C)3

50–55

60

60

50

[5, 8, 12]

Temp. stability (30 min, 60°C)

NA4

NA

405

705

[7, 8, 12]

  1. 1T. reesei VTT-D-86271 (Rut C-30)
  2. 2More than 60% of maximal activity retained.
  3. 3Optimum temperature was assayed at temperatures ranging from 20°C to 90°C for 10 min.
  4. 4NA, not available.
  5. 540% or 70% of total activity retained.
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BMC Biotechnology

ISSN: 1472-6750

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