Figure 3

Characterization of the recombinant Xyn2. (A) Effect of temperature on the activity of Xyn2. (B) Effect of pH on the activity of Xyn2. (C) The temperature stability of Xyn2 was determined after preincubating the enzyme in the absence of the substrate for 30 min at 50, 60, and 70°C. (D) The thermostability of Xyn2 at different temperatures was determined by preincubating the enzyme at these temperatures in the absence of substrate for 5, 10, 15, 20, 25, and 30 min before measuring its activity. The xylanase activity prior to the preincubations at different temperature was taken as 100%.