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Table 3 Summary of baculovirus purification from hemolymph for 25 number of silkworm larvae harvested at different post-injection

From: High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization

Harvesting time (p.i.) Volume (ml) Titer (pfu/ml) Virus (pfu) Protein (mg/ml) Virus/protein (pfu/mg) Recovery (%) Purification (fold)
4d        
Hemolymph 16 2.03E+08 3.25E+09 34.5 5.88E+06 100 1
Concentrated virus after Sephacryl 0.9 1.35E+08 1.22E+08 0.087 1.55E+09 4 264
4.5d        
Hemolymph 16 4.22E+08 6.75E+09 34.9 1.21E+07 100 1
Concentrated virus after Sephacryl 0.9 9.96E+07 8.96E+07 0.237 4.20E+08 1 35
5d        
Hemolymph 12 8.29E+08 9.95E+09 43.2 1.91E+07 100 1
Concentrated virus after Sephacryl 0.6 4.90E+08 2.94E+08 0.659 7.43E+08 3 39