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Table 3 Summary of baculovirus purification from hemolymph for 25 number of silkworm larvae harvested at different post-injection

From: High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization

Harvesting time (p.i.)

Volume (ml)

Titer (pfu/ml)

Virus (pfu)

Protein (mg/ml)

Virus/protein (pfu/mg)

Recovery (%)

Purification (fold)

4d

       

Hemolymph

16

2.03E+08

3.25E+09

34.5

5.88E+06

100

1

Concentrated virus after Sephacryl

0.9

1.35E+08

1.22E+08

0.087

1.55E+09

4

264

4.5d

       

Hemolymph

16

4.22E+08

6.75E+09

34.9

1.21E+07

100

1

Concentrated virus after Sephacryl

0.9

9.96E+07

8.96E+07

0.237

4.20E+08

1

35

5d

       

Hemolymph

12

8.29E+08

9.95E+09

43.2

1.91E+07

100

1

Concentrated virus after Sephacryl

0.6

4.90E+08

2.94E+08

0.659

7.43E+08

3

39