Synthesis of sialoglycopolypeptide for potentially blocking influenza virus infection using a rat α2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae

Table 3 Inhibition of SNA lectin hemagglutination by the artificial sialoglycopolypeptide as glycoprotein mimetics

Entry compounds	kDa	NS^d (%)	Sia^h (%)	IC₅₀ⁱ (nM)
γ-Polyglutamic acid (γ-PGA)	990^a	-	-	N.E.^j
Asialoglycopolypeptide	2100^b	40^e (33^f)	-	N.E.
α2,6-Sialoglycopolypeptide	2800^b	0	40 (39)	0.94
Fetuin (Sialoglycoprotein)	48^c	N.D.^g	(13.5)	730

^a Provided by Meiji Seika Kaisha, Ltd. (Tokyo, Japan).
^b Estimated from degree of substitution of asialo- or sialo-sugar derivatives based on DP of γ-PGA as 100%, which was calculated from ¹H NMR data at 25°C.
^c Neutral sugar derivatives substituted
^d Degree of substitution of asialo- or sialo-sugar derivatives based on DP of γ-PGA as 100%, which was calculated from ¹H NMR data at 25°C.
^e Degree of substitution of asialo- or sialo-sugar derivatives based on DP of γ-PGA as 100%, which was calculated from two types of HPLC analys (ABEE and DMB methods). All data normalized to those of ¹H NMR.
^f Not determined
^g Sialyl sugar derivatives substituted (= Sia contents)
^h Minimum concentrations required for complete inhibition of hemagglutination
ⁱ No effect means that inhibitory effect was not observed up to 200 nM of γ-PGA or asialoglycopolypeptide

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