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Table 2 Kinetic parameter for transfer reaction of acceptor substrates

From: Synthesis of sialoglycopolypeptide for potentially blocking influenza virus infection using a rat α2,6-sialyltransferase expressed in BmNPV bacmid-injected silkworm larvae

Sugar moiety

K m (mM)

V max (mM/min)

k cat (s-1)a

kcat/K m (mM-1s-1)

LacNAc β-Rb

0.92

0.17

1.13

1.23

Lactose β-R

2.80

0.013

0.087

0.031

  1. a k cat = Vmax/E, where E denote amount of enzyme.
  2. b R = 5-(5-Dimetylaminonaphthalene-1-sulfonyl-2-(2-aminoethoxy))ethyl