Table 2 The primers used in RT-PCR.
From: Construction of siRNA/miRNA expression vectors based on a one-step PCR process
SPOP-F | 5' GTCCTCCACCTCCGGCAGAA 3' |
SPOP-R | 5' GGATTGCTTCAGGCGTTTGC 3' |
P53-F | 5' ATGGAGGAGCCGCAGTCAGA 3' |
P53-R | 5' GTCTGAGTCAGGCCCTTCTGTCTT 3' |
EGFP-F | 5' ATGGTGAGCAAGGGCGAGGA 3' |
EGFP-R | 5' TTACTTGTACAGCTCGTCCATGCCG 3' |
FIRE-F | 5' GCCCAGCGCCATTCTACCCACTCG 3' |
FIRE-R | 5' TGCCGCCCTTCTTGGCCTTAATG 3' |
actin-F | 5' GGGAGAGCGGGAAATCGTGCGTGA 3' |
actin-R | 5' GATGGAGTTGAAGGTAGTTTCGTG 3' |