Figure 3
From: Construction of siRNA/miRNA expression vectors based on a one-step PCR process
Schematic diagram of the one-step PCR process. (D) shows the target structure: hU6 promoter-sense-loop-anti-sense-TTTTTT-SV40 polyA. The two panes indicate the primer sequences. The primer sequences are shown in (A). PCR then yields the linear vector shown in (B). It is obvious that a homologous region on each side, as shown in (C). Product transformation into chemically competent E. coli cells yields the structure shown in (D).