Figure 2
From: Construction of siRNA/miRNA expression vectors based on a one-step PCR process
Main procedures for the one-step PCR process. First, an appropriate PCR system and program should be adopted (A). Then the PCR products need to be authenticated and purified (B). After testing and purification, the PCR products can be transformed into chemically competent E. coli (C). Finally, positive clones (Db) are easily selected because negative clones (Da) exhibit green fluorescence under UV light.