Figure 1
From: Construction of siRNA/miRNA expression vectors based on a one-step PCR process
Illustrations of the features of pGsilG (A) and CMV-Gmir30/155G (B). pGsilG is constructed based on the shRNA expression vector pGenesil1.0. A GFP cassette is inserted between hU6 promoter and SV40 polyA. CMV-Gmir30/155G is constructed based on T-vector. A miRNA expression cassette (CMV IE-EGFP-mir30/155 5' flank-GFP cassette-mir30/155 3' flank-SV40 polyA) is inserted in MCS. EGFP driven by CMV IE promoter is fused with GFP cassette and this feature will turn to the result of EGFP-miRNA fusion structure.