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Table 2 Protocol of experiments for testing accuracy of the model

From: Integrated application of uniform design and least-squares support vector machines to transfection optimization

Sequence Number

Number of Cells (× 105)

Amount of plasmid (μg)

Amount of LipofectAMINE (μL)

Expression ratio of GFP (%)

Fitted Values

(%)

A

1.50

0.30

0.60

57.25

50.07

B

1.60

0.36

0.72

63.56

58.98

C

1.70

0.42

0.84

69.73

67.57

D

1.80

0.48

0.96

77.64

75.13

E

1.90

0.54

1.08

83.47

80.97

F

2.00

0.60

1.20

86.42

84.47

G

2.10

0.66

1.32

92.32

85.12

H

2.20

0.72

1.44

85.19

82.59

I

2.30

0.78

1.56

81.29

76.73

J

2.40

0.84

1.68

73.61

67.62