Table 1 Protocol of experiments for generating the model
Sequence Number | Number of Cells (× 105)* | Amount of plasmid (μg) * | Amount of LipofectAMINE (μL) * | Expression ratio of GFP % | Fitted Values % |
|---|---|---|---|---|---|
A | 11.0 | 100.50 | 80.88 | 64.74 | 64.50 |
B | 21.1 | 50.25 | 40.56 | 45.58 | 45.72 |
C | 31.2 | 140.70 | 111.12 | 68.37 | 68.06 |
D | 41.3 | 20.10 | 131.28 | 19.60 | 20.25 |
E | 51.4 | 120.60 | 10.32 | 30.61 | 31.04 |
F | 61.5 | 70.35 | 60.72 | 56.16 | 56.09 |
G | 71.6 | 80.40 | 151.44 | 75.24 | 74.80 |
H | 81.7 | 40.20 | 101.04 | 48.78 | 48.85 |
I | 91.8 | 150.75 | 50.64 | 59.19 | 59.06 |
J | 101.9 | 30.15 | 20.40 | 38.23 | 38.51 |
K | 112.0 | 110.55 | 141.36 | 82.53 | 81.94 |
L | 122.1 | 10.05 | 70.80 | 13.49 | 14.26 |
M | 132.2 | 130.65 | 90.96 | 71.67 | 71.29 |
N | 142.3 | 90.45 | 30.48 | 51.96 | 51.97 |
O | 152.4 | 60.30 | 121.20 | 61.85 | 61.67 |