Comparison of ELISA results, high resolution melt curve analysis (HRMA) and restriction digestion analysis. Shown in the top row of the small square boxes are representative clones from groups as assigned by HRMA. The different colours indicate the different groups, and a cross indicates these samples could not be analysed because of a low quality melt curve. The second row of boxes indicates the ELISA results. A white box represents a negative ELISA result and an orange box indicates a positive ELISA result. The corresponding restriction digestion pattern for each sample is shown in the lower panel. HRMA identifies an additional group in the ELISA-positive clones (top panel sample 12,13, 18, 20 and 21) not identified by fingerprint analysis.