Suitability of the silica/DODAB/antigen system to induce improved cytokines production. Cytokine responses generated by SiO2/DODAB/18 kDa-hsp and Al(OH)3/18 kDa-hsp. Release of IL-10 (A), IL-12 (B), IL-13 (C) and IFN-γ (D) from inguinal and periaortic lymph node cells isolated from BALB/c mice immunized with 15 μg of 18 kDa-hsp M. leprae administered in silica/DODAB or Al(OH)3. Lymph node cells were isolated six days after immunization and re-stimulated in vitro with 250 μg/ml of 18 kDa-hsp or 2.5 μg/mL of ConA for 48 hours. The cytokine levels were measured by sandwich kit enzyme-linked immunosorbent assay (ELISA) as described in materials and methods. The limit of detection was 156 pg/mL for IL-10 and IL-12, and 78 pg/mL for IL-13 and IFN-γ respectively.