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Table 1 Confocal imaging parameters.

From: Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates

Fluorophore Filter Set Pinhole Diameter Laser (nm)/Transmission (%) Detector Gain
DAPI BP 420–480 74 405/4.1 766
GFP BP 505–530 82 488/46.6 765
Alexa 555 BP 570–620 94 543/16.6 542
Qdot 655 BP 635–675 106 488/34.7 618
Qdot 705 BP 680–730 106 488/34.7 578
Qdot 800 LP 745 82 488/46.6 649
  1. Details of the imaging parameters used during the collection of data for Fig. 2. Amplifier offset and amplifier gain settings were constant throughout (0.1 and 1, respectively).