Expanding the multicolor capabilities of basic confocal microscopes by employing red and near-infrared quantum dot conjugates

Table 1 Confocal imaging parameters.

Fluorophore	Filter Set	Pinhole Diameter	Laser (nm)/Transmission (%)	Detector Gain
DAPI	BP 420–480	74	405/4.1	766
GFP	BP 505–530	82	488/46.6	765
Alexa 555	BP 570–620	94	543/16.6	542
Qdot 655	BP 635–675	106	488/34.7	618
Qdot 705	BP 680–730	106	488/34.7	578
Qdot 800	LP 745	82	488/46.6	649

Details of the imaging parameters used during the collection of data for Fig. 2. Amplifier offset and amplifier gain settings were constant throughout (0.1 and 1, respectively).

ISSN: 1472-6750