Alexa 555 and Qdot 655 secondary antibodies produce images of similar quality. Fibroblasts were stained with anti-α-tubulin antibody, followed by secondary antibodies coupled to Alexa 555 (A) or Qdot 655 (B). Insets show magnification of outlined region. Images in (A) and (B) are maximal projections of deconvolved z-stack data. Images in (C) and (D) show single xy planes of the inset regions from (A) and (B), prior to deconvolution.