Filter configuration and data acquisition strategy for 6-parameter imaging experiments. Graphs show emission spectra of organic dye and Qdot reagents employed in this study. The spectra are grouped with the laser line used for excitation (405 nm, top; 488 nm, middle; 543 nm, bottom). Colored bands indicate the approximate range of emissions transmitted by the filters (see also Table 1). In the middle graph, the red line and red axis indicates the approximate quantum efficiency of the Hamamatsu 6357 PMT through the displayed wavelength range. Spectra are based on manufacturer's supplied data from Invitrogen (DAPI and Qdots) and Clontech (EGFP), and PMT quantum efficiency information is based on technical data from Hamamatsu.